Review



continuous-flow chambers with polycarbonate filters  (AVESTIN Inc)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    AVESTIN Inc continuous-flow chambers with polycarbonate filters
    ATP and glycerol 3-phosphate formation with continuous substrate feed. (A, B) Vesicles are retained by the <t>polycarbonate</t> filter (50 nm pore diameter). (A) Schematic of the experimental setup; 2.78 mg/mL of vesicles are added to the vesicle compartment and sampled over time. A flow of 50 mM KPi pH 7.0 is applied to the feed compartment. (B) LC-MS data normalized for dilution reveals that the total lipid composition is constant over time ( n = 2; error bars represent s.e.m.). (C, D) Metabolites equilibrate through the polycarbonate filter. (C) Schematic of the experimental setup. A metabolite gradient is imposed by applying 5 mM l -arginine/ l -ornithine/ l -citrulline in 50 mM KPi pH 7.0 to either of the chamber compartments. (D) HPLC data normalized for dilution reveal metabolite equilibration through the polycarbonate filter ( n = 4; error bars represent s.e.m.). Equilibration occurs at the same rate for both compartments. (E, F) ATP and glycerol 3-phosphate synthesis with a continuous l -arginine and glycerol feed. (E) Schematic of the experimental setup. The vesicles are applied to the vesicle compartment and the substrates are fed through the feed compartment. (F) Online ATP/ADP readout measured with PercevalHR ( n = 3; error bars represent s.e.m.).
    Continuous Flow Chambers With Polycarbonate Filters, supplied by AVESTIN Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/continuous-flow+chambers+with+polycarbonate+filters/continuous+flow+chambers+with+polycarbonate+filters/pmc09295154-226-6-8
    Average 90 stars, based on 1 article reviews
    continuous-flow chambers with polycarbonate filters - by Bioz Stars, 2026-08
    90/100 stars

    Images

    1) Product Images from "ATP Recycling Fuels Sustainable Glycerol 3-Phosphate Formation in Synthetic Cells Fed by Dynamic Dialysis"

    Article Title: ATP Recycling Fuels Sustainable Glycerol 3-Phosphate Formation in Synthetic Cells Fed by Dynamic Dialysis

    Journal: ACS Synthetic Biology

    doi: 10.1021/acssynbio.2c00075

    ATP and glycerol 3-phosphate formation with continuous substrate feed. (A, B) Vesicles are retained by the polycarbonate filter (50 nm pore diameter). (A) Schematic of the experimental setup; 2.78 mg/mL of vesicles are added to the vesicle compartment and sampled over time. A flow of 50 mM KPi pH 7.0 is applied to the feed compartment. (B) LC-MS data normalized for dilution reveals that the total lipid composition is constant over time ( n = 2; error bars represent s.e.m.). (C, D) Metabolites equilibrate through the polycarbonate filter. (C) Schematic of the experimental setup. A metabolite gradient is imposed by applying 5 mM l -arginine/ l -ornithine/ l -citrulline in 50 mM KPi pH 7.0 to either of the chamber compartments. (D) HPLC data normalized for dilution reveal metabolite equilibration through the polycarbonate filter ( n = 4; error bars represent s.e.m.). Equilibration occurs at the same rate for both compartments. (E, F) ATP and glycerol 3-phosphate synthesis with a continuous l -arginine and glycerol feed. (E) Schematic of the experimental setup. The vesicles are applied to the vesicle compartment and the substrates are fed through the feed compartment. (F) Online ATP/ADP readout measured with PercevalHR ( n = 3; error bars represent s.e.m.).
    Figure Legend Snippet: ATP and glycerol 3-phosphate formation with continuous substrate feed. (A, B) Vesicles are retained by the polycarbonate filter (50 nm pore diameter). (A) Schematic of the experimental setup; 2.78 mg/mL of vesicles are added to the vesicle compartment and sampled over time. A flow of 50 mM KPi pH 7.0 is applied to the feed compartment. (B) LC-MS data normalized for dilution reveals that the total lipid composition is constant over time ( n = 2; error bars represent s.e.m.). (C, D) Metabolites equilibrate through the polycarbonate filter. (C) Schematic of the experimental setup. A metabolite gradient is imposed by applying 5 mM l -arginine/ l -ornithine/ l -citrulline in 50 mM KPi pH 7.0 to either of the chamber compartments. (D) HPLC data normalized for dilution reveal metabolite equilibration through the polycarbonate filter ( n = 4; error bars represent s.e.m.). Equilibration occurs at the same rate for both compartments. (E, F) ATP and glycerol 3-phosphate synthesis with a continuous l -arginine and glycerol feed. (E) Schematic of the experimental setup. The vesicles are applied to the vesicle compartment and the substrates are fed through the feed compartment. (F) Online ATP/ADP readout measured with PercevalHR ( n = 3; error bars represent s.e.m.).

    Techniques Used: Liquid Chromatography with Mass Spectroscopy



    Similar Products

    continuous-flow chambers with polycarbonate filters  (AVESTIN Inc)
    90
    AVESTIN Inc continuous-flow chambers with polycarbonate filters
    ATP and glycerol 3-phosphate formation with continuous substrate feed. (A, B) Vesicles are retained by the <t>polycarbonate</t> filter (50 nm pore diameter). (A) Schematic of the experimental setup; 2.78 mg/mL of vesicles are added to the vesicle compartment and sampled over time. A flow of 50 mM KPi pH 7.0 is applied to the feed compartment. (B) LC-MS data normalized for dilution reveals that the total lipid composition is constant over time ( n = 2; error bars represent s.e.m.). (C, D) Metabolites equilibrate through the polycarbonate filter. (C) Schematic of the experimental setup. A metabolite gradient is imposed by applying 5 mM l -arginine/ l -ornithine/ l -citrulline in 50 mM KPi pH 7.0 to either of the chamber compartments. (D) HPLC data normalized for dilution reveal metabolite equilibration through the polycarbonate filter ( n = 4; error bars represent s.e.m.). Equilibration occurs at the same rate for both compartments. (E, F) ATP and glycerol 3-phosphate synthesis with a continuous l -arginine and glycerol feed. (E) Schematic of the experimental setup. The vesicles are applied to the vesicle compartment and the substrates are fed through the feed compartment. (F) Online ATP/ADP readout measured with PercevalHR ( n = 3; error bars represent s.e.m.).
    Continuous Flow Chambers With Polycarbonate Filters, supplied by AVESTIN Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/continuous-flow+chambers+with+polycarbonate+filters/continuous+flow+chambers+with+polycarbonate+filters/pmc09295154-226-6-8
    Average 90 stars, based on 1 article reviews
    continuous-flow chambers with polycarbonate filters - by Bioz Stars, 2026-08
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    ATP and glycerol 3-phosphate formation with continuous substrate feed. (A, B) Vesicles are retained by the polycarbonate filter (50 nm pore diameter). (A) Schematic of the experimental setup; 2.78 mg/mL of vesicles are added to the vesicle compartment and sampled over time. A flow of 50 mM KPi pH 7.0 is applied to the feed compartment. (B) LC-MS data normalized for dilution reveals that the total lipid composition is constant over time ( n = 2; error bars represent s.e.m.). (C, D) Metabolites equilibrate through the polycarbonate filter. (C) Schematic of the experimental setup. A metabolite gradient is imposed by applying 5 mM l -arginine/ l -ornithine/ l -citrulline in 50 mM KPi pH 7.0 to either of the chamber compartments. (D) HPLC data normalized for dilution reveal metabolite equilibration through the polycarbonate filter ( n = 4; error bars represent s.e.m.). Equilibration occurs at the same rate for both compartments. (E, F) ATP and glycerol 3-phosphate synthesis with a continuous l -arginine and glycerol feed. (E) Schematic of the experimental setup. The vesicles are applied to the vesicle compartment and the substrates are fed through the feed compartment. (F) Online ATP/ADP readout measured with PercevalHR ( n = 3; error bars represent s.e.m.).

    Journal: ACS Synthetic Biology

    Article Title: ATP Recycling Fuels Sustainable Glycerol 3-Phosphate Formation in Synthetic Cells Fed by Dynamic Dialysis

    doi: 10.1021/acssynbio.2c00075

    Figure Lengend Snippet: ATP and glycerol 3-phosphate formation with continuous substrate feed. (A, B) Vesicles are retained by the polycarbonate filter (50 nm pore diameter). (A) Schematic of the experimental setup; 2.78 mg/mL of vesicles are added to the vesicle compartment and sampled over time. A flow of 50 mM KPi pH 7.0 is applied to the feed compartment. (B) LC-MS data normalized for dilution reveals that the total lipid composition is constant over time ( n = 2; error bars represent s.e.m.). (C, D) Metabolites equilibrate through the polycarbonate filter. (C) Schematic of the experimental setup. A metabolite gradient is imposed by applying 5 mM l -arginine/ l -ornithine/ l -citrulline in 50 mM KPi pH 7.0 to either of the chamber compartments. (D) HPLC data normalized for dilution reveal metabolite equilibration through the polycarbonate filter ( n = 4; error bars represent s.e.m.). Equilibration occurs at the same rate for both compartments. (E, F) ATP and glycerol 3-phosphate synthesis with a continuous l -arginine and glycerol feed. (E) Schematic of the experimental setup. The vesicles are applied to the vesicle compartment and the substrates are fed through the feed compartment. (F) Online ATP/ADP readout measured with PercevalHR ( n = 3; error bars represent s.e.m.).

    Article Snippet: The continuous-flow chambers with 50 nm polycarbonate filters (Avestin) were preequilibrated with 50 mM KPi pH 7.0.

    Techniques: Liquid Chromatography with Mass Spectroscopy